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KMID : 1134120110140010020
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Journal of Breast Cancer
2011 Volume.14 No. 1 p.20 ~ p.27
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Role of Estrogen Receptor-α in the Regulation of Claudin-6 Expression in Breast Cancer Cells
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Yafang Liu
Qiong Wu
Yue Ren
Xiaoming Xu
Lina Yu
Mingzi Zhang
Ting Zhang
Yulin Li
Chengshi Quan
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Abstract
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Purpose: In our previous studies we showed that upregulating claudin-6 (CLDN6) expression may contribute to preventing breast cancer, and that 17β-estradiol induces a concentration- and time-related effect on CLDN6 mRNA and protein expression in MCF-7 cells. However, the mechanisms of 17β-estradiol regulation of CLDN6 are still unclear. We determined the role of estrogen receptors in the regulation of CLDN6 expression in human breast cancer tissues and a cell line.
Methods: CLDN6, estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) expression in breast cancer tissues were examined using immunohistochemistry. The human breast cancer cell line, MCF-7, which expresses ERα but not ERβ was used. CLDN6 and ERα expression were measured by reverse transcriptase-PCR, Western blotting and immunofluorescent staining. Treatments with propyl pyrazole triol (PPT) and ICI 182, 780 (ICI) were performed.
Results: The results revealed that CLDN6 expression was related to ERα in breast cancer tissues (p=0.033). PPT, an ERα-selective ligand, upregulated CLDN6 expression at 10-5 mol/L after 24 hours. The effect of PPT on regulating CLDN6 expression in MCF-7 cells was blocked by ICI.
Conclusion: These findings suggest that Erα reulates CLDN6 expression in breast cancer tissues and that 17β-estradiol induces CLDN6 expression through an ERα pathway in MCF-7 cells.
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KEYWORD
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Breast carcinoma, Claudins, Estrogen, Estrogen receptor alpha, Tight junctions
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